CD43 antibody (AA 272-400)

Catalog No. ABIN7601042

The Mouse Monoclonal anti-CD43 antibody (Clone 4I3) (ABIN7601042) specifically detects CD43 in FACS, WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CD43 antibody (AA 272-400) (ABIN7601042)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 4I3

Product Details anti-CD43 Antibody

Binding Specificity: AA 272-400

Purpose: Anti-CD43/SPN Antibody (monoclonal, 4I3)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human CD43 shares 72%,73% amino acid (aa) sequence identity with mouse,rat CD43,respectively.

Characteristics: Anti-CD43/SPN Antibody.(monoclonal, 4I3)-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequence identity with mouse and rat CD43, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: SPN

Background:

Background: CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

Gene Full Name: sialophorin

Molecular Weight: 115 kDa

Gene ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity