Vimentin (VIM) antibody (HRP)

Antigen: Vimentin (VIM)

Synonyms: FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime

Clonality: Monoclonal (RV202)

Host: Mouse

Reactivity: Dog (Canine), Chicken, Goat, Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus), Pig (Porcine), Zebrafish

Conjugate: HRP

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN1042494

Additional Information

Alternative name: Vimentin

Isotype: IgG1

Clone: RV202

Description: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Characteristics: Cytoskeletal,Tumour marker

Specificity: RV202-HRP reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-HRP is suitable for immunoblotting, immunocytochemistry on cell cultures and cytospins, as well as immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:10.

Comments:

RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Application Details

Application Notes: RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

Purification: Purified

Buffer: PBS containing 0,1% BSA, 0.09% sodium azide.

Storage: Store at 4°C, or in small aliquots at -20°C.

Research Area: Cancer, Cytoskeleton, Immunology, Neurology, Stem Cells, Lineage Markers, Neural Stem Cell marker, Cell/Tissue Markers

Restrictions: For Research Use only

Publications

Product: Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).