CD82 (CD83) antibody

Antigen: CD82 (CD83)

Synonyms: BL11, HB15, R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27

Clonality: Monoclonal (C33)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Functional Studies (Func)

Catalog no.: ABIN1043024

Additional Information

Alternative name: CD82

Gene ID: 3732

Immunogen: C91/PL (human HTLV-1+ T cell line)

Isotype: IgG2a

Clone: C33

Description: The mouse monoclonal antibody C33 recognizes CD82 (KAI1), a member of the tetraspanin family, which forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.

Specificity: The antibody C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

Comments:

CD and Related Antigens (Human)

Application Details

Purity: Purified

Buffer: Phosphate buffered saline (PBS) pH 7.4

Storage: Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.

Research Area: Cancer, Transcription Factors, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Product: Fukudome, Furuse, Imai et al.: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, 1992 (PubMed).

Imai, Kakizaki, Nishimura et al.: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, 1995 (PubMed).

Imai, Yoshie: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, 1994 (PubMed).

Ueda, Ichikawa, Tamaru et al.: "Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer." in: The American journal of pathology, Vol. 149, Issue 5, pp. 1435-40, 1996 (PubMed).

Escola, Kleijmeer, Stoorvogel et al.: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, 1998 (PubMed).