IL-6 antibody (HRP)

Details for Product No. ABIN1043799
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Antigen
Synonyms Il-6, ILg6, Ifnb2, BSF2, HGF, HSF, IFNB2, IL-6, CHIL-6, il6
Reactivity
Human
(354), (160), (102), (28), (27), (24), (15), (10), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host
Rabbit
(292), (168), (103), (18), (7), (3), (1)
Clonality
Polyclonal
Conjugate
HRP
(69), (26), (13), (11), (9), (9), (9), (9), (9), (9), (9), (9), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA)
(354), (339), (87), (80), (73), (68), (64), (45), (39), (36), (35), (27), (18), (16), (15), (12), (12), (10), (10), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN1043799
361.90 $
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Immunogen This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG
Specificity This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.  A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
Characteristics This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µL by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purification purified
Background IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Synonyms: HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
Gene ID 3569
NCBI Accession NP_000591
UniProt P05231
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer, Receptors
Application Notes This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µL by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.1 mg/ml
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide!
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
anti-IL-6 antibody (HRP) Western Blot of Peroxidase conjugated Rabbit anti-Human IL-6 antibody. Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none.
Product cited in: Novick: "Current concepts in the management of renovascular hypertension and ischemic renal failure." in: American journal of kidney diseases : the official journal of the National Kidney Foundation, Vol. 13, Issue 6 Suppl 1, pp. 33-7, 1989 (PubMed).

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