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Interleukin 6 antibody (IL6) (HRP)

Details for Product anti-IL6 Antibody No. ABIN1043799, Supplier: Log in to see
Antigen
  • Il-6
  • ILg6
  • Ifnb2
  • BSF2
  • HGF
  • HSF
  • IFNB2
  • IL-6
  • CHIL-6
  • il6
Reactivity
Human
441
174
98
23
10
8
7
7
7
7
5
4
3
3
2
2
1
Host
Rabbit
341
205
153
37
9
7
2
Clonality
Polyclonal
Conjugate
This Interleukin 6 antibody is conjugated to HRP
102
37
17
16
11
6
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
Application
Neutralization (Neut), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
473
444
110
107
99
62
44
43
39
32
28
25
22
17
14
12
12
8
6
5
4
4
4
4
4
4
3
2
2
2
1
1
1
1
1
Supplier
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Immunogen This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG
Specificity This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.  A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
Characteristics This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µL by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purification purified
Alternative Name IL-6 (IL6 Antibody Abstract)
Background IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Synonyms: HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
Gene ID 3569
NCBI Accession NP_000591
UniProt P05231
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer, Receptors
Pathways
Application Notes This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µL by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.1 mg/ml
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide!
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Interleukin 6 antibody (IL6)  (HRP) (ABIN1043799) Western Blot of Peroxidase conjugated Rabbit anti-Human IL-6 antibody. Lane 1: Human ...
Product cited in: Novick: "Current concepts in the management of renovascular hypertension and ischemic renal failure." in: American journal of kidney diseases : the official journal of the National Kidney Foundation, Vol. 13, Issue 6 Suppl 1, pp. 33-7, 1989 (PubMed).