IL-6 antibody (HRP)

Antigen: IL-6

Protein Type: Recombinant

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: HRP

Application: ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Catalog no.: ABIN1043799

Additional Information

Gene ID: 3569

NCBI Acession: NP_000591.1

UniProt: P05231

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
Immunogen Type: Recombinant Protein

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Isotype: IgG

Description: This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Synonyms: HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF.

Characteristics: IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Specificity: This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.  A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.

Comments:

Gene Name: IL6

Application Details

Application Notes: ELISA Dilution: 1:10,000-1:50,000.
Immunohistochemistry Dilution: 1:500-1:2,500.
Western Blot Dilution: 1:1,000-1:5,000.

Handling Advice: Do NOT add Sodium Azide!

Concentration: 1.1 mg/ml

Purification: purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: Expiration date is one (1) year from date of opening.

Research Area: Cytokines, Immunology, Virology, Inflammation, Cancer, Receptors

Restrictions: For Research Use only

Images

Western Blot of Peroxidase conjugated Rabbit anti-Human IL-6 antibody. Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none.

Publications

Product: Novick: "Current concepts in the management of renovascular hypertension and ischemic renal failure." in: American journal of kidney diseases : the official journal of the National Kidney Foundation, Vol. 13, Issue 6 Suppl 1, pp. 33-7, 1989 (PubMed).