Interleukin 17A (IL17A) antibody (HRP)

Antigen: Interleukin 17A (IL17A)

Synonyms: IL17, CTLA8, IL-17, IL-17A, Il17, Ctla8, Ctla-8, IL17F, IL17A, ChIL-17

Protein Type: Recombinant

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus), Mouse (Murine)

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN1043841

Additional Information

Alternative name: IL-17A

Gene ID: 301289

NCBI Acession: Q61453.1

UniProt: Q61453

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.
Immunogen Type: Recombinant Protein

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Isotype: IgG

Description: This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 30.0 kDa in size corresponding to rat IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Synonyms: Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8, HRP.

Characteristics: Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and rat IL-17A.

Specificity: This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Comments:

Gene Name: IL17A

Application Details

Handling Advice: Do NOT add Sodium Azide!

Purification: purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Publications

Product: Rouvier, Luciani, Mattéi et al.: "CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5445-56, 1993 (PubMed).