Adrenergic Receptor, alpha 1d (ADRA1D) (Cytoplasmic Domain) antibody

Details for Product No. ABIN1048311, Supplier: Log in to see
Antigen
  • Adra-1
  • Adra1
  • Adra1a
  • Gpcr8
  • Spr8
  • [a]1d
  • alpha1D-AR
  • Adrd1
  • ADRA1
  • ADRA1A
  • ADRA1R
  • ALPHA1
  • DAR
  • dJ779E11.2
  • adrenergic receptor, alpha 1d
  • adrenoceptor alpha 1D
  • Adra1d
  • ADRA1D
Epitope
Cytoplasmic Domain
4
3
3
2
2
2
2
2
2
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Monkey, Pig (Porcine), Rabbit
35
12
9
4
4
3
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
35
Clonality
Polyclonal
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
19
13
13
8
6
4
2
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Brand IHC-plus™
Immunogen Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human ADRA1D. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Bovine, Dog, Bat, Panda, Horse, Rabbit, Pig (100%), Mouse, Rat, Sheep, Hamster (90%).

Type of Immunogen: Synthetic peptide
Specificity Human ADRA1D. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Bovine, Dog, Bat, Panda, Horse, Rabbit, Pig (100%) Mouse, Rat, Sheep, Hamster (90%).
Purification Immunoaffinity purified
Alternative Name ADRA1D (ADRA1D Antibody Abstract)
Background Name/Gene ID: ADRA1D
Subfamily: Adrenergic
Family: GPCR

Synonyms: ADRA1D, Alpha 1d, Alpha-1D adrenergic receptor, ADRA1R, Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ALPHA1, Alpha-1D-adrenergic receptor, Alpha-adrenergic receptor 1a, Alpha1delta-AR, DAR, ADRA1, Adrenoceptor alpha 1D, Alpha-1A adrenergic receptor, DJ779E11.2
Gene ID 146
Application Notes Approved: IHC-P (1 - 3 μg/mL)
Comment

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Assay Procedure

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Adrenergic Receptor, alpha 1d (ADRA1D) (Cytoplasmic Domain) antibody (ABIN1048311) Anti-ADRA1D antibody ABIN1048311 IHC staining of human prostate. Immunohistochemistr...
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