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SLC44A2 antibody (Internal Region)

The Rabbit Polyclonal anti-SLC44A2 antibody has been validated for IHC and IHC (p). It is suitable to detect SLC44A2 in samples from Human.
Catalog No. ABIN1049339

Quick Overview for SLC44A2 antibody (Internal Region) (ABIN1049339)

Target

See all SLC44A2 Antibodies
SLC44A2 (Solute Carrier Family 44, Member 2 (SLC44A2))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This SLC44A2 antibody is un-conjugated

Application

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Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    Internal Region

    Brand

    IHC-plus™

    Specificity

    Human SLC44A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

    Predicted Reactivity

    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Gibbon, Monkey, Marmoset (94%) Orangutan, Galago, Dog (88%) Rat, Hamster, Elephant, Panda, Horse, Pig, Guinea pig (81%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic 16 amino acid peptide from internal region of human SLC44A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Monkey, Marmoset (94%), Orangutan, Galago, Dog (88%), Rat, Hamster, Elephant, Panda, Horse, Pig, Guinea pig (81%).

    Type of Immunogen: Synthetic peptide
  • Application Notes

    Approved: IHC, IHC-P (10 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS, less than 0.1 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    SLC44A2 (Solute Carrier Family 44, Member 2 (SLC44A2))

    Alternative Name

    SLC44A2 / CTL2

    Background

    Name/Gene ID: SLC44A2
    Subfamily: Choline transporter-like
    Family: Transporter

    Synonyms: SLC44A2, CTL2, PP1292

    Gene ID

    57153

    Pathways

    SARS-CoV-2 Protein Interactome
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