PPAR alpha (N-Term) antibody

Antigen: PPAR alpha

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN105798

Additional Information

Alternative name: PPAR alpha (NT)

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human PPAR alpha. Peptide sequence: M-V-D-T-E-S-P-I-C-P-L-S-P-L-E-A-D-D

Format: Liquid (sterile filtered)

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Application Details

Application Notes: Recommended Dilutions: This product has been assayed by ELISA against 0.1 ug of the immunizing peptide. A 1:8,000 to 1:32,000 dilution of the antibody is recommended for this assay. Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Transcription Factors, Metabolism, Receptors

Restrictions: For Research Use only

Publications

Publications: Zak, Gelebart, Lai: "Fenofibrate induces effective apoptosis in mantle cell lymphoma by inhibiting the TNFalpha/NF-kappaB signaling axis." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, 2010 (PubMed).