PPARA antibody (AA 1-468)

Catalog No. ABIN1944858

Product Details anti-PPARA Antibody

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Binding Specificity: AA 1-468

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This PPARA antibody is generated from a mouse immunized with a recombination protein from the human region of human PPARA.

Clone: 1331CT894-186-143

Isotype: IgG1 kappa

Application Details

Application Notes: IF: 1:25. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-PPARA antibody (ABIN1944858)

Cho, Lee, Choi, Yang, Tae Hong, Kim, Yoon: "Optimization of an enzyme-linked immunosorbent assay to screen ligand of Peroxisome proliferator-activated receptor alpha." in: Immunopharmacology and immunotoxicology, Vol. 31, Issue 3, pp. 459-67, (2009) (PubMed).

Kobayashi, Kodani, Nozawa, Endo, Sawasaki: "DNA-binding profiling of human hormone nuclear receptors via fluorescence correlation spectroscopy in a cell-free system." in: FEBS letters, Vol. 582, Issue 18, pp. 2737-44, (2008) (PubMed).

Tugwood, Aldridge, Lambe, Macdonald, Woodyatt: "Peroxisome proliferator-activated receptors: structures and function." in: Annals of the New York Academy of Sciences, Vol. 804, pp. 252-65, (1997) (PubMed).

Mukherjee, Jow, Noonan, McDonnell: "Human and rat peroxisome proliferator activated receptors (PPARs) demonstrate similar tissue distribution but different responsiveness to PPAR activators." in: The Journal of steroid biochemistry and molecular biology, Vol. 51, Issue 3-4, pp. 157-66, (1995) (PubMed).

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: PPARA (PPARA Products)

Synonyms: NR1C1 antibody, PPAR antibody, PPARalpha antibody, hPPAR antibody, LOC100136415 antibody, PPARALPHA antibody, nr1c1 antibody, xPPARalpha antibody, xppara antibody, 4933429D07Rik antibody, AW742785 antibody, Nr1c1 antibody, PPAR-alpha antibody, Ppar antibody, peroxisome proliferator activated receptor alpha antibody, peroxisome proliferator-activated receptor alpha antibody, peroxisome proliferator activated receptor alpha L homeolog antibody, PPARA antibody, LOC100136415 antibody, ppara.L antibody, Ppara antibody, ppara antibody

Background: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.

Molecular Weight: 52225

Gene ID: 5465

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C