Streptavidin antibody (Texas Red (TR))

Details for Product No. ABIN107095
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Antigen
Reactivity
Streptomyces avidinii (S. avidinii)
(80), (11), (8), (2)
Host
Rabbit
(50), (49), (2)
Clonality
Polyclonal
Conjugate
Texas Red (TR)
(9), (8), (7), (5), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunomicroscopy (IM), Flow Cytometry (FACS)
(66), (56), (27), (21), (9), (9), (8), (8), (7), (7), (6), (5), (3)
Pubmed 1 reference available
Quantity 20 mg
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Catalog No. ABIN107095
3,316.50 $
Plus shipping costs $45.00

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Streptavidin (Streptomyces avidinii)
Immunogentype:Native
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Background Streptavidin Antibody Texas Red™ Conjugated antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Research Area Epitope Tags, Tags/Labels, Enzymes
Application Notes Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 10 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Streptavidin antibody (Texas Red (TR)) Rabbit anti Streptavidin (200-4195 lot 23495) and Biotin conjugated Rabbit anti-trypsin inhibitor antibody (200-4679 lot 6594) were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit ( 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc System
General Titus, Haugland, Sharrow et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).

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