Streptavidin antibody (Texas Red (TR))

Antigen: Streptavidin

Protein Type: Native

Clonality: Polyclonal

Host: Rabbit

Reactivity: Streptomyces avidinii (S. avidinii)

Conjugate: Texas Red (TR)

Application: Please enquire

Catalog no.: ABIN107095

Additional Information

Immunogen: Streptavidin (Streptomyces avidinii)

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Isotype: IgG

Description: Streptavidin Antibody Texas Red™ Conjugated antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Application Details

Application Notes: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Fluorochrome Protein Value: 2.3
IF Microscopy Dilution: 1:500-1:2,500

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free. Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. 

Research Area: Epitope Tags, Tags/Labels, Enzymes

Restrictions: For Research Use only

Images

Rabbit anti Streptavidin (200-4195 lot 23495) and Biotin conjugated Rabbit anti-trypsin inhibitor antibody (200-4679 lot 6594) were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit ( 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc System

Publications

Titus JA, Haugland R, Sharrow SO et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: J Immunol Methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).