Streptavidin antibody (FITC)

Catalog No. ABIN5596792

Product Details anti-Streptavidin Antibody (FITC)

Target: Streptavidin

Reactivity: Streptomyces avidinii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Streptavidin antibody is conjugated to FITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Cross-Reactivity (Details): No reaction was observed against Avidin.

Purification: Anti-Streptavidin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Streptavidin.

Immunogen:

Immunogen: Streptavidin (Streptomyces avidinii)

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 2.3

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: Suitable for immunomicroscopy, FLISA, fluorescent WB, flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

FLISA Dilution: User Optimized

IF Microscopy Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Streptavidin antibody (FITC) (ABIN5596792)

Min, Park, Kim, Moon, Lee, Kim, Moon, Yang, Lee, Kim, Cho, Park, Lee, Lee, Park: "Tim-4 functions as a scavenger receptor for phagocytosis of exogenous particles." in: Cell death & disease, Vol. 11, Issue 7, pp. 561, (2020) (PubMed).

Target Details for Streptavidin

Target: Streptavidin

Abstract: Streptavidin Products

Background:

Synonyms: FITC, Streptavidin Fluorescein, Anti-Streptavidin FITC antibody

Background: Anti-Streptavidin Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.