Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
At least two isoforms of SLC35D3 are known to exist, this antibody will recognize both isoforms. SLC35D3 antibody is predicted to not cross-react with SLC35D1 or SLC35D2.
Purification
Affinity chromatography purified via peptide column
Immunogen
SLC35D3 antibody was raised against a AA 17 synthetic peptide near the carboxy terminus of human SLC35D3.
SLC35D3 antibody can be used for detection of SLC35D3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL. Optimal dilutions for each application to be determined by the researcher.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage
4 °C/-20 °C
Storage Comment
SLC35D3 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year.
Expiry Date
12 months
Target
SLC35D3
(Solute Carrier Family 35, Member D3 (SLC35D3))
FRCL1 antibody, bA55K22.3 antibody, 6230421J19Rik antibody, Frcl1 antibody, solute carrier family 35, member D3 antibody, solute carrier family 35 member D3 antibody, Slc35d3 antibody, SLC35D3 antibody, slc35d3 antibody
Background
Synonyms: Solute carrier family 35 member D3, FRCL1
The solute carrier family SLC35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The novel protein SLC35D3 is highly homologous to SLC35D1 and SLC35D2, both of which transport UDP-glucuronic acid and UDP-N-acetylgalactosamine, suggesting that SLC35D3 is also involved in the transport of nucleotide sugars. It has been suggested that SLC35D3 regulates platelet dense granules, lysosome-related organelles which contain high concentrations of several biologically important low molecular weight molecules necessary for normal blood homeostasis.