Keratin 3 antibody

Catalog No. ABIN108617

The Mouse Monoclonal anti-Keratin 3 antibody has been validated for WB, IF, ICC, IHC (p) and FACS. It is suitable to detect Keratin 3 in samples from Human, Rabbit and Cow. There are 4+ publications available.

Quick Overview for Keratin 3 antibody (ABIN108617)

Target: Keratin 3 (KRT3)

Reactivity: Human, Rabbit, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: AE5

Product Details anti-Keratin 3 Antibody

Purification: Protein G purified

Immunogen: Rabbit corneal epithelial keratins

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as guideline try at WB: 1/1000 - 1/3000. Predicted molecular weight: 65 kDa.Flow Cyt: Use 2 μg for 106 cells.

Comment:

Myeloma, fusion partners: P3 x 63 Ag8 myeloma cells,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References for anti-Keratin 3 antibody (ABIN108617)

Zhou, Long, Hsu, Liu, Chen, Slavin, Lin, Li, Tang, Yiu, Tuffaha, Mao: "Nanofiber-Reinforced Decellularized Amniotic Membrane Improves Limbal Stem Cell Transplantation in a Rabbit Model of Corneal Epithelial Defect." in: Acta biomaterialia, (2019) (PubMed).

Liao, Irvine, Macewen, Weed, Porter, Corden, Gibson, Moore, Smith, McLean, Moore: "Development of allele-specific therapeutic siRNA in Meesmann epithelial corneal dystrophy." in: PLoS ONE, Vol. 6, Issue 12, pp. e28582, (2011) (PubMed).

Rodrigues, Ben-Zvi, Krachmer, Schermer, Sun: "Suprabasal expression of a 64-kilodalton keratin (no. 3) in developing human corneal epithelium." in: Differentiation; research in biological diversity, Vol. 34, Issue 1, pp. 60-7, (1987) (PubMed).

Cooper, Sun: "Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression." in: The Journal of biological chemistry, Vol. 261, Issue 10, pp. 4646-54, (1986) (PubMed).

Target Details for Keratin 3

Target: Keratin 3 (KRT3)

Alternative Name: Cytokeratin 3

Background: Synonyms:
CK3 antibody,FLJ95909 antibody,K3 antibody,65 kDa cytokeratin antibody,CK 3 antibody,Cytokeratin3 antibody,Keratin 3 antibody,Keratin type II cytoskeletal 3 antibody,Keratin3 antibody,KRT 3 antibody,KRT3 antibody

Gene ID: 281267, 3850

OMIM: 148043

UniProt: P12035, Q29426