Formyl Peptide Receptor 2 (FPR2) antibody

Antigen: Formyl Peptide Receptor 2 (FPR2)

Synonyms: ALXR, HM63, FMLPX, FPR2A, FPRH1, FPRH2, FPRL1, LXA4R, FMLP-R-II, Fpr-rs2, MGC151443, E330010I07Rik, FPR2, Fprl1, ALX, Lxa4r, LXA4-R, Fpr-rs1, FMLP-R-I

Clonality: Monoclonal (GM1D6)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN108736

Additional Information

Alternative name: FPRL1

Immunogen: Genetic immunisation with cDNA encoding human Crisp3

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: GM1D6

Description: Human formyl peptide-receptor-like-1 (FPRL1) belongs to the large family of G-protein coupled receptors. More than 1000 human GPCR-genes were identified. GPCRs are characterized by seven transmembrane helices. In most cases, the extracellular N-terminal domain and the three extracellular loops are responsible for binding ligands and thereby determine receptor activity.FPRL1 shares 69% identity at the amino acid-level with FPR, the well-known formyl peptide receptor. FPLR1 has been reported as a low-affinity fMLF receptor and, therefore, has been proposed to function in host defence mechanisms due to its ability to bind formylated bacterial peptides. FPRL1 is expressed in astrocytes, neutrophils and monocytes. Since FPRL1 is expressed in neutrophils and monocytes and it was shown using another monoclonal antibody that chemokines can be potent and specific ligands (Elagoz et al., 2003) FPRL1 might have interesting functions in inflammatory pathways.

Specificity: GM1D6 recognises FPRL1 transiently expressed on the cell surface of transfected BOSC cells (Fig. 1).

Application Details

Application Notes: FACS: 1.2ug/10^6 cells, Cell-ELISA: 1:200 to 1:400

Storage: Store the antibody at 4°C. Avoid repeated freezing and thawing.

Research Area: Signaling, Immunology, Inflammation

Restrictions: For Research Use only

Images

Specificity testing of GM1D6. BOSC cells were transiently transfected with anexpression vector for FPRL1 as well as an irrelevant protein. Expression of the constructswas tested with anti-tagmonoclonal antibodies (B, blueand red curves). An irrelevant mo

Publications

Publications: Elagoz, Henderson, Babu et al.: "A truncated form of CKbeta8-1 is a potent agonist for human formyl peptide-receptor-like 1 receptor." in: British journal of pharmacology, Vol. 141, Issue 1, pp. 37-46, 2004 (PubMed).

Brancaleone, Dalli, Bena et al.: "Evidence for an anti-inflammatory loop centered on polymorphonuclear leukocyte formyl peptide receptor 2/lipoxin A4 receptor and operative in the inflamed microvasculature." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 8, pp. 4905-14, 2011 (PubMed).

Nadkarni, Cooper, Brancaleone et al.: "Activation of the Annexin A1 Pathway Underlies the Protective Effects Exerted by Estrogen in Polymorphonuclear Leukocytes." in: Arteriosclerosis, thrombosis, and vascular biology, 2011 (PubMed).