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UTF1 antibody (Undifferentiated Embryonic Cell Transcription Factor 1) (AA 148-214)

Details for Product anti-UTF1 Antibody No. ABIN1098126, Supplier: Log in to see
Antigen
  • AI505934
Epitope
AA 148-214
29
15
14
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
62
19
14
1
Host
Mouse
44
15
5
1
Clonality (Clone)
Monoclonal ()
Conjugate
This UTF1 antibody is un-conjugated
5
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
51
36
11
5
5
2
2
2
1
Supplier
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Immunogen Purified recombinant fragment of human UTF1 (AA 148-214) expressed in E. coli.
Clone 5B6
Isotype IgG1
Purification Purified
Alternative Name UTF1 (UTF1 Antibody Abstract)
Background Undifferentiated transcription factor-1 (UTF-1) is used as marker for the undifferentiated state of pluripotent stem cells.UTF1 is a chromatin-associated protein with core histone-like properties. UTF1 further acts as a transcriptional repressor and is required for proper differentiation of pluripotent cells.
Molecular Weight 36.4 kDa
Gene ID 8433
HGNC 8433
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-UTF1 antibody (Undifferentiated Embryonic Cell Transcription Factor 1) (AA 148-214) (ABIN1098126) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-UTF1 antibody (Undifferentiated Embryonic Cell Transcription Factor 1) (AA 148-214) (ABIN1098126) Figure 2: Flow cytometric analysis of K562 cells using UTF1 mouse mAb (green) and neg...
Western Blotting (WB) image for anti-UTF1 antibody (Undifferentiated Embryonic Cell Transcription Factor 1) (AA 148-214) (ABIN1098126) Figure 3: Western blot analysis using UTF1 mAb against human UTF1 recombinant protein...
Western Blotting (WB) image for anti-UTF1 antibody (Undifferentiated Embryonic Cell Transcription Factor 1) (AA 148-214) (ABIN1098126) Figure 4: Western blot analysis using UTF1 mAb against HEK293 (1) and UTF1 (AA: 148-2...
Product cited in: Reinisch, Mildner, Petzelbauer et al.: "Embryonic stem cell factors undifferentiated transcription factor-1 (UFT-1) and reduced expression protein-1 (REX-1) are widely expressed in human skin and may be involved in cutaneous ..." in: International journal of experimental pathology, Vol. 92, Issue 5, pp. 326-32, 2011 (PubMed).

Kooistra, Thummer, Eggen: "Characterization of human UTF1, a chromatin-associated protein with repressor activity expressed in pluripotent cells." in: Stem cell research, Vol. 2, Issue 3, pp. 211-8, 2011 (PubMed).