ACLY antibody (ATP Citrate Lyase) (AA 306-502)

Details for Product anti-ACLY Antibody No. ABIN1098143, Supplier: Log in to see
Antigen
  • ACLY
  • DDBDRAFT_0205389
  • DDBDRAFT_0235360
  • DDB_0205389
  • DDB_0235360
  • ACL
  • BcDNA:LD21334
  • CG8322
  • DmATPCL
  • Dmel\\CG8322
  • anon-WO0140519.179
  • dATPCL
  • l(2)01466
  • l(2)k09217
  • n(2)k09217
  • ATPCL
  • CLATP
  • A730098H14Rik
  • AW538652
  • Clatp
  • acly
  • cb722
  • zgc:92008
  • ATP citrate lyase
  • ATP-citrate synthase
  • citrate synthase, mitochondrial
  • atp-citrate synthase
  • ATP citrate lyase a
  • ACLY
  • acly
  • ATPCL
  • AaeL_AAEL004297
  • CpipJ_CPIJ010291
  • Bm1_26245
  • ACL
  • Acly
  • aclya
Epitope
AA 306-502
18
17
5
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
121
89
88
26
7
7
5
3
3
3
3
3
2
2
1
1
Host
Mouse
82
42
Clonality (Clone)
Monoclonal ()
Conjugate
This ACLY antibody is un-conjugated
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
94
48
43
35
34
31
29
26
5
3
3
2
1
Options
Supplier
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Supplier Product No.
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Immunogen Purified recombinant fragment of human ACLY (AA 306-502 ) expressed in E. Coli.
Clone 5F8D11
Isotype IgG1
Purification Purified
Alternative Name ACLY (ACLY Antibody Abstract)
Background ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. , , ,
Molecular Weight 125 kDa
Gene ID 47
HGNC 47
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:50, FCM: 1:200 - 1:400
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Supplier Images
ELISA image for anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (ABIN1098143) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (ABIN1098143) Figure 2: Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (ABIN1098143) Figure 3: Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green) . Bl...
Immunohistochemistry (IHC) image for anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (ABIN1098143) Figure 4: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissue...
Immunohistochemistry (IHC) image for anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (ABIN1098143) Figure 5: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues...
Western Blotting (WB) image for anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (ABIN1098143) Figure 6: Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6...
Western Blotting (WB) image for anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (ABIN1098143) Figure 7: Western blot analysis using ACLY mAb against human ACLY recombinant protein...
Product cited in: Chu, Lin, Hendel, Kulpa, Brownsey, Johnson: "ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells." in: The Journal of biological chemistry, Vol. 285, Issue 42, pp. 32606-15, 2010 (PubMed).

Beckner, Fellows-Mayle, Zhang, Agostino, Kant, Day, Pollack: "Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas." in: International journal of cancer. Journal international du cancer, Vol. 126, Issue 10, pp. 2282-95, 2010 (PubMed).

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