Ankyrin G antibody (C-Term)

Catalog No. ABIN1098195

Product Details anti-Ankyrin G Antibody

Target: Ankyrin G (ANK3) (Ankyrin 3, Node of Ranvier (Ankyrin G) (ANK3))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ankyrin G antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Purpose: Anti-Ankyrin-G Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Ankyrin-G.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein corresponding to ~1,000 C-terminal amino acids of human Ankyrin G (accession no. NP_066267.2).

Clone: S106-20

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-2 μg/mL. A band of >200 kDa is detected.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: rat brain membranes.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Target Details for Ankyrin G

Target: Ankyrin G (ANK3) (Ankyrin 3, Node of Ranvier (Ankyrin G) (ANK3))

Alternative Name: Ankyrin-G (ANK3 Products)

Synonyms: ANK3 antibody, ankyrin-G antibody, 2900054D09Rik antibody, AI314020 antibody, Ank-3 antibody, AnkG antibody, Ankyrin-3 antibody, Ankyrin-G antibody, ANKYRIN-G antibody, ankyrin 3 antibody, ankyrin 3, node of Ranvier (ankyrin G) antibody, ankyrin 3, epithelial antibody, ANK3 antibody, legA6 antibody, PB000272.02.0 antibody, PY00018 antibody, ank3 antibody, Ank3 antibody

Background: Ankyrin-3,In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368). {PubMed:17974005}., [Isoform 5]: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin. {PubMed:17974005}.,Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and for at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers, and ion transporters in the plasma membrane.,Cytoplasm, cytoskeleton, Cell projection, axon, Cell membrane, sarcolemma, Cell junction, synapse, postsynaptic cell membrane, Lysosome, Cell membrane, sarcolemma, T-tubule,ANK-3, Ankyrin-G

UniProt: Q12955

Pathways: Synaptic Membrane, Maintenance of Protein Location, Skeletal Muscle Fiber Development