ALPL antibody (N-Term)

Catalog No. ABIN1105292

Product Details anti-ALPL Antibody

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: EKEKDPKYWR DQAQE

Specificity: This antibody detects Alkaline phosphatase / ALPL at N-term. No cross reactivity with other proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide corresponding to a sequence at the N-terminal of Human ALPL (21-35)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg Sodium Azide

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: Alkaline Phosphatase / ALPL (ALPL Products)

Synonyms: ALP antibody, iap antibody, alpl antibody, AP-TNAP antibody, APTNAP antibody, HOPS antibody, TNAP antibody, TNSALP antibody, Akp-2 antibody, Akp2 antibody, PHOA antibody, TNS-AP antibody, alkaline phosphatase, liver/bone/kidney L homeolog antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase, intestinal, gene 2 antibody, alkaline phosphatase, liver/bone/kidney pseudogene antibody, alpl.L antibody, ALPL antibody, alpi.2 antibody, LOC100031702 antibody, Alpl antibody

Background: Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts, the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.Synonyms: AP-TNAP, Alkaline phosphatase, Alkaline phosphatase liver/bone/kidney isozyme, TNSALP, tissue-nonspecific isozyme

Gene ID: 249

NCBI Accession: NP_000469

UniProt: P05186