Neurofilament, Heavy Polypeptide (NEFH) (C-Term) antibody

Details for Product No. ABIN1108414, Supplier: Log in to see
Antigen
  • NF-H
  • NF200
  • Nfh
  • mKIAA0845
  • NFH
  • NEFH
  • neurofilament heavy polypeptide
  • neurofilament, heavy polypeptide
  • MCYG_06231
  • Nefh
  • NEFH
Epitope
C-Term
32
18
18
15
9
3
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
407
340
319
240
201
99
80
79
56
54
54
52
31
27
26
25
17
3
2
2
2
1
Host
Mouse
375
54
18
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
14
13
12
12
12
12
12
12
12
12
12
12
11
11
10
10
9
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
257
227
150
149
122
121
68
56
13
12
8
2
2
2
1
Options
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Available images

Immunogen C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
Clone NF-200
Isotype IgG1
Specificity This antibody reacts to Neurofilament H (200 kDa).
Purification Affinity chromatography
Alternative Name Neurofilament H (200 kD) (NEFH Antibody Abstract)
Background Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 Kd (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT)Synonyms: (Neuronal Marker), 200 kDa neurofilament protein, KIAA0845, NEFH, NF-H, NFH, Neurofilament triplet H protein, heavy polypeptide
Gene ID 4744
NCBI Accession NP_066554
UniProt P12036
Research Area Cytoskeleton, Cell/Tissue Markers
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Reconstitution Restore with 1.2 % sodium acetate or neutral PBS
Concentration 0,1 mg/mL
Buffer 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (IHC) image for anti-Neurofilament, Heavy Polypeptide (NEFH) (C-Term) antibody (ABIN1108414) anti-Neurofilament, Heavy Polypeptide (NEFH) (C-Term) antibody
Background publications Brownlees, Ackerley, Grierson, Jacobsen, Shea, Anderton, Leigh, Shaw, Miller: "Charcot-Marie-Tooth disease neurofilament mutations disrupt neurofilament assembly and axonal transport." in: Human molecular genetics, Vol. 11, Issue 23, pp. 2837-44, 2002 (PubMed).

Lees, Shneidman, Skuntz, Carden, Lazzarini: "The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it." in: The EMBO journal, Vol. 7, Issue 7, pp. 1947-55, 1988 (PubMed).