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CD5 antibody (CD5 Molecule) (APC)

Details for Product anti-CD5 Antibody No. ABIN1111110, Supplier: Log in to see
Antigen
  • CD5
  • LEU1
  • Ly-1
  • Ly-12
  • Ly-A
  • Lyt-1
  • T1
Reactivity
Human
808
255
139
73
54
40
39
35
30
29
28
26
25
21
15
3
1
1
1
Host
Mouse
952
220
124
Clonality (Clone)
Monoclonal ()
Conjugate
This CD5 antibody is conjugated to APC
155
135
105
55
31
28
25
24
20
20
18
18
18
18
18
18
14
14
10
10
8
8
6
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
1039
408
306
278
236
180
165
121
117
15
12
11
11
8
8
8
5
5
4
2
1
1
1
1
1
1
Supplier
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Immunogen Human acute lymphoblastic leukemia (ALL) T cells
Clone L17F12
Isotype IgG2a
Specificity The mouse monoclonal antibody L17F12 reacts with CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name CD5 (CD5 Antibody Abstract)
Background CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Gene ID 921
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Product cited in: Dunphy, Tang: "The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases." in: Archives of pathology & laboratory medicine, Vol. 131, Issue 5, pp. 748-54, 2007 (PubMed).

Gong, Lagoo, Peters, Horvatinovich, Benz, Buckley: "Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma." in: American journal of clinical pathology, Vol. 116, Issue 6, pp. 893-7, 2001 (PubMed).

McAlister, Davis, Pfuhl, Driscoll: "NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments." in: Protein engineering, Vol. 11, Issue 10, pp. 847-53, 1999 (PubMed).

Shuster, Falletta, Pullen, Crist, Humphrey, Dowell, Wharam, Borowitz: "Prognostic factors in childhood T-cell acute lymphoblastic leukemia: a Pediatric Oncology Group study." in: Blood, Vol. 75, Issue 1, pp. 166-73, 1990 (PubMed).

Engleman, Warnke, Fox, Dilley, Benike, Levy: "Studies of a human T lymphocyte antigen recognized by a monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 3, pp. 1791-5, 1981 (PubMed).