KRT20 antibody (FITC)

Catalog No. ABIN111170

The Mouse Monoclonal anti-KRT20 antibody has been validated for IF, IHC (p), FACS and IHC (fro). It is suitable to detect KRT20 in samples from Human, Rat and Pig. There is 1 publication available.

Quick Overview for KRT20 antibody (FITC) (ABIN111170)

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Human, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT20 antibody is conjugated to FITC

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: KS20-10

Product Details anti-KRT20 Antibody (FITC)

Specificity: This Monoclonal antibody represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide Recognized: Protein IT (keratin K20, Mr 46 000, also designated Cytokeratin 20). Tested Reactivities on Cultured Cell Lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.

No Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Species reactivity (tested):Human, Porcine (Pig) and Rat.

Purification: Affinity Chromatography

Immunogen: Electrophoretically purified Keratin K 20 from Human intestinal mucosa.

Isotype: IgG1

Application Details

Application Notes: FACS. Cytological Material. Immunohistochemistry on Frozen Sections. . Immunohistochemistry on Paraffin sections (Only after microwave treatment). Working Dilution: 1/10-1/20 in PBS, PH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT or over night at 2-8 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-KRT20 antibody (FITC) (ABIN111170)

Mușină, Zlei, Mentel, Scripcariu, Ștefan, Aniţei, Filip, Radu, Gavrilescu, Panuţa, Buna-Arvinte, Cordun, Predescu, Scripcariu, Huţanu: "Evaluation of circulating tumor cells in colorectal cancer using flow cytometry." in: The Journal of international medical research, Vol. 49, Issue 9, pp. 300060520980215, (2021) (PubMed).

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: Cytokeratin 20

Background: Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT

Gene ID: 54474

NCBI Accession: NP_061883

UniProt: P35900