KRT20 antibody

Catalog No. ABIN335316

Cited in 1 publication. The Mouse Monoclonal anti-KRT20 antibody (Clone MKs20-8) (ABIN335316) specifically detects KRT20 in WB, ICC, IP and IHC (fro). The antibody is reactive with Various Species samples.

Quick Overview for KRT20 antibody (ABIN335316)

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MKs20-8

Product Details anti-KRT20 Antibody

Purpose: Mouse anti Cytokeratin 20

Specificity: MKs20-8 may be used as a marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin, it may also be used as a sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma).

Characteristics: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Purification: culture supernatant

Immunogen: MKs20-8 is a Mouse monoclonal IgG2a antibody derived by fusion of Mouse myeloma cells with spleen cells from a Balb/c Mouse immunized with electrophoretically purified Keratin K20 from Human intestinal mucosa.

Isotype: IgG2a

Application Details

Application Notes: MKs20-8 is suitable for immunoprecipitation, immunoblotting, immunocytochemistry on fixed cells, and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 1 mL of culture supernatant of monclonal antibody containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

References for anti-KRT20 antibody (ABIN335316)

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, (1990) (PubMed).

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: Cytokeratin 20 / Keratin K20

UniProt: P35900