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EpCAM antibody (FITC)

EPCAM Reactivity: Human IF, IHC (p), IHC (fro) Host: Mouse Monoclonal HEA125 FITC
Catalog No. ABIN111179
  • Target See all EpCAM (EPCAM) Antibodies
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    Reactivity
    • 283
    • 75
    • 35
    • 2
    • 1
    • 1
    Human
    Host
    • 150
    • 143
    • 10
    • 1
    Mouse
    Clonality
    • 186
    • 114
    • 2
    Monoclonal
    Conjugate
    • 154
    • 20
    • 17
    • 13
    • 9
    • 9
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This EpCAM antibody is conjugated to FITC
    Application
    • 218
    • 177
    • 123
    • 111
    • 90
    • 33
    • 26
    • 24
    • 23
    • 20
    • 19
    • 16
    • 14
    • 10
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).Polypeptide reacting:
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    Affinity Chromatography on Protein A
    Immunogen
    HT-29 colon carcinoma cell line
    Clone
    HEA125
    Isotype
    IgG1
    Top Product
    Discover our top product EPCAM Primary Antibody
  • Application Notes
    Immunofluorescence Microscopy. Cell Sorting, Cytological Material. Immunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. With Paraffin embedded sections, protease pretreatment is required prior to antibodyapplication. Working Dilution: Dilute at least 1/10 with PBS, pH 7.4 for Immunohistochemicalapplication. Incubation Time: 1 h at RT.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    3.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    Alternative Name
    CD326 / EPCAM / TACSTD1 (EPCAM Products)
    Synonyms
    DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody
    Background
    Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
    Gene ID
    4072
    NCBI Accession
    NP_002345
    UniProt
    P16422
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