Epithelial Cell Adhesion Molecule (EPCAM) antibody (FITC)

Antigen: Epithelial Cell Adhesion Molecule (EPCAM)

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Clonality: Monoclonal (HEA125)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Catalog no.: ABIN111179

Additional Information

Alternative name: CD326 / EPCAM / TACSTD1

UniProt: P16422

Immunogen: HT-29 colon carcinoma cell line

Cross-Reactivity: Human

Isotype: IgG1

Clone: HEA125

Description: Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigenhas been identified independently by a number of groups, and has been known by avariety of names. Several monoclonal antibodies have been raised against EpCAM, manyof which have been described as tumour specific molecules on carcinomas. EpCAM is aType 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cellsby the majority of epithelial tissues, with the exception of adult squamous epithelium andsome specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, withexpression being increased in tumour cells, and de novo expression being seen indysplastic squamous epithelium.

Characteristics: Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2,M4S1, MIC18, Tumor-associated calcium signal transducer 1, Major gastrointestinaltumor-associated protein GA733-2, Epithelial glycoprotein 314,Adenocarcinoma-associated antigen, KS 1/4 antigen

Specificity: The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM(also described as 17-1A antigen or EPG34). This antigen is widely expressed on cells ofepithelial origin and tumors derived therefrom. HEA125 represents an excellent marker todiscriminate epithelial from mesothelial structures. The antigen has been detected in allcarcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularlystrong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein(Ep-CAM). Species: Human. Others not tested.

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Application Details

Application Notes: Immunofluorescence Microscopy. Cell Sorting, Cytological Material. Immunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. With Paraffin embedded sections, protease pretreatment is required prior to antibodyapplication. Working Dilution: Dilute at least 1/10 with PBS, pH 7.4 for Immunohistochemicalapplication. Incubation Time: 1 h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 3.0 mg/ml

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Joplin, Strain, Neuberger: "Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture." in: The Journal of pathology, Vol. 162, Issue 3, pp. 255-60, 1991 (PubMed).

Hastka, Pfiester: "[Cell marker for the differential diagnosis of malignant mesotheliomas]" in: Der Pathologe, Vol. 9, Issue 4, pp. 245-7, 1989 (PubMed).

Edwards: "Heterogeneous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies." in: British journal of cancer, Vol. 51, Issue 2, pp. 149-60, 1985 (PubMed).

Joplin, Strain, Neuberger: "Immuno-isolation and culture of biliary epithelial cells from normal human liver." in: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 25, Issue 12, pp. 1189-92, 1990 (PubMed).

Momburg, Moldenhauer, Haemmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

Racila, Euhus, Weiss et al.: "Detection and characterization of carcinoma cells in the blood." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4589-94, 1998 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).