Epithelial Cell Adhesion Molecule (EPCAM) antibody (FITC)

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Antigen
Synonyms
DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, EGP, Ep-CAM, CD326, Egp314, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40, sb:cb6, tacstd, wu:fj17g02, zgc:11030 ... show more
DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, EGP, Ep-CAM, CD326, Egp314, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40, sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2 show less
Reactivity
Human
(223), (52), (39), (17), (17), (17), (2)
Host
Mouse
(173), (58), (12)
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
(17), (8), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(127), (114), (76), (50), (45), (38), (34), (33), (29), (20), (5), (5), (3), (2), (2), (2)
Pubmed 11 references available
Catalog no. ABIN111179
Quantity 0.25 mL
Price
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Immunogen HT-29 colon carcinoma cell line
Clone HEA125
Isotype IgG1
Specificity The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep- CAM). Species Reactivity: Tested: Human.
Purification Affinity Chromatography on Protein A
Alternative Name CD326 / EPCAM / TACSTD1
Background Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
Alternate names: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Gene ID 4072
NCBI Accession NP_002345.2
UniProt P16422
Research Area Cancer
Application Notes Immunofluorescence Microscopy. Cell Sorting, Cytological Material. Immunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. With Paraffin embedded sections, protease pretreatment is required prior to antibodyapplication. Working Dilution: Dilute at least 1/10 with PBS, pH 7.4 for Immunohistochemicalapplication. Incubation Time: 1 h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 3.0 mg/mL
Buffer 0.09% Sodium Azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Simon, Podolsky, Moldenhauer et al.: "Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 7, pp. 2755-9, 1990 (PubMed).

Joplin, Strain, Neuberger: "Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture." in: The Journal of pathology, Vol. 162, Issue 3, pp. 255-60, 1991 (PubMed).

Moldenhauer, Momburg, Möller et al.: "Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker." in: British journal of cancer, Vol. 56, Issue 6, pp. 714-21, 1988 (PubMed).

Edwards: "Heterogeneous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies." in: British journal of cancer, Vol. 51, Issue 2, pp. 149-60, 1985 (PubMed).

Momburg, Moldenhauer, Hämmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

Winter, Nagtegaal, van Krieken et al.: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, 2003 (PubMed).

Metzgeroth, Mantz, Kuhn et al.: "Reliable identification of small cell lung cancer in cytological specimens by immunocytology." in: Onkologie, Vol. 30, Issue 6, pp. 311-5, 2007 (PubMed).

General Hastka, Pfiester: "[Cell marker for the differential diagnosis of malignant mesotheliomas]." in: Der Pathologe, Vol. 9, Issue 4, pp. 245-7, 1989 (PubMed).

Joplin, Strain, Neuberger: "Immuno-isolation and culture of biliary epithelial cells from normal human liver." in: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 25, Issue 12, pp. 1189-92, 1990 (PubMed).

Racila, Euhus, Weiss et al.: "Detection and characterization of carcinoma cells in the blood." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4589-94, 1998 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).

Hosts (173), (58), (12)
Reactivities (223), (52), (39), (17), (17), (17), (2)
Applications (127), (114), (76), (50), (45), (38), (34), (33), (29), (20), (5), (5), (3), (2), (2), (2)
Conjugates (17), (8), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Epitopes (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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