EpCAM antibody (Cytoplasmic Domain)

Catalog No. ABIN6939974

The Mouse Monoclonal anti-EpCAM antibody is suitable to detect EpCAM in samples from Human, Dog and Cat. It has been validated for IHC.

Quick Overview for EpCAM antibody (Cytoplasmic Domain) (ABIN6939974)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: EGP40-1110

Product Details anti-EpCAM Antibody

Binding Specificity: AA 289-314, Cytoplasmic Domain

Purpose: Ep-CAM / CD326 (Cytoplasmic Domain) (Epithelial Marker) Antibody

Specificity: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment from cytoplasmic domain (around aa 289-314) of human EpCAM (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: Colon or ovarian tissues (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: TACSTD1

Background:

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, Membrane Component, Chromosome 17, Surface Marker-1 (M17S1), REG-1, Reggie-1, Reggie-2

MCF-7 or HT29 cells. Human lung or ovarian carcinoma (IHC).

Molecular Weight: 40-43kDa

Gene ID: 4072, 542050

UniProt: P16422