KLKB1 antibody

Catalog No. ABIN111208

This Mouse Monoclonal antibody specifically detects KLKB1 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for KLKB1 antibody (ABIN111208)

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLKB1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: 901

Product Details anti-KLKB1 Antibody

Specificity: Recognizes two variants of Prekallikrein and its activation products Kallikrein (88 kDa and 85 kDa), the complexes formed by kallikrein with its endogenous inhibitors C1 inhibitor, alpha-2-macroglobulin and Antithrombin III, and 45 kDa prekallikrein/kallikrein fragment(s). Recognizes Prekallikrein and its activation products in Human, Chimpanzee, Rhesus, and Baboon plasmas. The epitope for this antibody, located on the prekallikrein/kallikrein heavy chain, is involved in the interaction between prekallikrein and factor XIIa. This antibody inhibits prekallikrein activation in Human and Rhesus plasmas by approximately 60-80 % and 55 %, respectively. Does not cross-react with tissue Kallikrein

Purification: Protein G chromatography.

Immunogen: Purified Human prekallikrein.

Isotype: IgG1

Application Details

Application Notes: Suitable for use in ELISA and Western blot. Prekallikrein activation may occur with repeated freezing/thawing (3x or more) of plasmasamples.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.6 mg/mL (OD280 nm)

Buffer: PBS, pH 7.4 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KLKB1

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Background: Plasma Kallikrein 1B is a serine proteinase of the S1 family It is secreted by the liver, kidney, adrenal gland and placenta. The zymogen is referred to as prekallikrein (PK) and is activated by Factor XIIa to an active two chain form, plasma kallikrein. In Fletcher syndrome plasma kallikrein is deficient, resulting in a prolonged partial thromboplastin time (PT).Synonyms: Fletcher factor, Plasma kallikrein

Gene ID: 3818

NCBI Accession: NP_000883

UniProt: P03952

Pathways: Complement System, Brown Fat Cell Differentiation