Microtubule Associated Protein 2 (MAP2) antibody

Antigen: Microtubule Associated Protein 2 (MAP2)

Synonyms: MAP2A, MAP2B, MAP2C, DKFZp686I2148, MAP2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, map2, MGC145864

Clonality: Monoclonal (AP20)

Host: Mouse

Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN111291

Additional Information

Characteristics: Synonyms: Microtubule-associated protein 2, MAP2, Neuronal Marker

Alternative name: MAP-2

Swiss-Prot: P11137

Immunogen: Bovine brain Microtubule Associated Protein 2.

Isotype: IgG1  (Matching secondary antibodies)

Clone: AP20

Description: MAP2 is the major microtubule associated protein of brain tissue. There are three forms ofMAP2, two are similarily sized with apparent molecular weights of 280 kDa (MAP2a andMAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression ofMAP2 is developmentally regulated and its multiple forms arise by alternative splicing of asingle gene.

Specificity: Thisantibody recognizes (Mr300 kDa) MAP-2 protein from bovine brain. The antibodystrongly cross-reacts with MAP-2 from Human, Rat, Mouse, Xenopus, quail and Chickenbrains. BM2472 reacts with the High Molecular Weight forms (2a & 2b) of MAP-2 but notwith the Low Molecular Weight (70 kDa) form (2c). It reacts with dendrites and cell bodies of neurons.

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1/500. Immunofluorescence: 1/500. Western Blotting: 1-2 µg/ml for 2hrs at RT. Immunohistology (Formalin/Paraffin): 1-2 µg/ml for 30 min at RT. Staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml (OD280)

Buffer: 0.01M phosphate buffered saline (PBS) pH 7.2 containing 1% BSA asstabilizer and 0.09% Sodium Aazide as a preservative.

Storage: Store the antibody undiluited at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cytoskeleton

Restrictions: For Research Use only

Images

Formalin-fixed, paraffin-embedded human brain stained with Microtubule-Associated Protein 2a,b Ab (Cat. ABIN111291) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of the neurons and dendrites.

Publications

Publications: Lewis, Ivanov, Lee et al.: "Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau." in: Nature, Vol. 342, Issue 6249, pp. 498-505, 1990 (PubMed).

Peng, Binder, Black: "Biochemical and immunological analyses of cytoskeletal domains of neurons." in: The Journal of cell biology, Vol. 102, Issue 1, pp. 252-62, 1986 (PubMed).