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MAP2 antibody (Microtubule-Associated Protein 2) Primary Antibody

MAP2 Reactivity: Human, Mouse, Pig ELISA, ICC, IHC (fro), IHC (p), IP, mIHC, WB Host: Mouse Monoclonal MT-08 unconjugated
Pubmed (1 reference) Independent Validation (1 validation)
Catalog No. ABIN125739
$384.05
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0.1 mg
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  • Target See all MAP2 Antibodies
    MAP2
    Reactivity
    • 162
    • 107
    • 96
    • 32
    • 24
    • 7
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Pig
    Host
    • 132
    • 47
    • 13
    • 2
    Mouse
    Clonality
    • 148
    • 45
    Monoclonal
    Conjugate
    • 97
    • 18
    • 15
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    This MAP2 antibody is un-conjugated
    Application
    • 116
    • 73
    • 59
    • 39
    • 39
    • 37
    • 32
    • 31
    • 23
    • 20
    • 18
    • 11
    • 4
    • 3
    • 2
    • 1
    • 1
    ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Multiplex Immunohistochemistry (mIHC), Western Blotting (WB)
    Specificity
    The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab).
    Cross-Reactivity (Details)
    Human, Porcine, Mouse
    Purification
    Purified by protein-A affinity chromatography
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Microtubule protein (bovine brain) enriched for kinesin
    Clone
    MT-08
    Isotype
    IgG1
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Validation #104333 (Multiplex Immunohistochemistry)
    'Independent Validation' Badge
    by
    Akoya Biosciences
    No.
    #104333
    Date
    04/20/2021
    Antigen
    MAP2
    Lot Number
    536958
    Method validated
    Multiplex Immunohistochemistry
    Positive Control

    Fresh frozen mouse olfactory bulb

    Negative Control

    Unlabeled control (mouse fresh frozen)

    Notes

    Passed. The anti-MAP2 antibody ABIN125739 specifically labels structural elements of neurons. Staining can be observed in neuronal cell bodies, axons and occasionally in proximal dendritic processes.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN125739
    Secondary Antibody
    Full Protocol
    • Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
    • Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
    • Conjugation of the anti-MAP2 antibody ABIN125739 to an oligo barcode used to bind oligo-bound fluorophore ATTO 550.
    Experimental Notes
    • No signal was detected in unlabeled specimens.

    • Specific staining of MAP2 was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

    • Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Dzijak, Yildirim, Kahle, Novák, Hnilicová, Venit, Hozák: "Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner." in: PLoS ONE, Vol. 7, Issue 1, pp. e30529, (2012) (PubMed).

  • Target
    MAP2
    Alternative Name
    MAP2ab (MAP2 Products)
    Synonyms
    MAP2A, MAP2B, MAP2C, MAP2R, Mtap2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, zgc:103474, zgc:113974, microtubule associated protein 2, microtubule-associated protein 2, microtubule associated protein 2 S homeolog, MAP2, Map2, map2.S, map2
    Background
    MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.
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