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8-Hydroxyguanosine (8-OHDG) antibody

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Antigen
Reactivity
Chemical
27
2
1
1
1
1
Host
Mouse
15
14
Clonality (Clone)
Monoclonal ()
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
22
14
8
4
3
3
2
2
2
1
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Immunogen 8-Hydroxyguanosine (OH8G)-BSA and -casein conjugates.
Clone 15A3
Isotype IgG2b
Purification Protein G Chromatography.
Alternative Name 8-Hydroxy-Guanosine / 8-OHG
Target Type Chemical
Background 8 Hydroxyguanosine (80G) is a modified base which occurs in DNA as a result of oxidative stress. 8 Hydroxy-2'deoxyguanosine (8OHdG) has become a sensitive marker of oxidative damage in cellular DNA and has been reported to be excreted in the urine. Levels of 8OHdG have been shown to increase on exposure to ionizing radiation and tobacco smoke.Synonyms: 8-Hydroxy-2'-deoxyguanosine, 8-Hydroxyguanine, 8-Hydroxyguanosine, 8-OFdG, 8-OHG, 8OG, 8OHG, 8OHdG
Application Notes Suitable for use in ELISA and Immunohistochemistry (PLP-fixed sections) to detect themarkers of oxidative damage to DNA and RNA. May also be used on Immunoaffinity columns to isolate oxidative DNA damage adductsfrom biological fluids.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL (OD 280 nm)
Buffer TBS, pH 7.4 with 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Background publications Salganik, Albright, Rodgers, Kim, Zeisel, Sivashinskiy, Van Dyke: "Dietary antioxidant depletion: enhancement of tumor apoptosis and inhibition of brain tumor growth in transgenic mice." in: Carcinogenesis, Vol. 21, Issue 5, pp. 909-14, 2000

Cui, Holmes, Liu: "Oxidative damage to the c-fos gene and reduction of its transcription after focal cerebral ischemia." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 1164-74, 1999

Nunomura, Perry, Pappolla, Wade, Hirai, Chiba, Smith: "RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 6, pp. 1959-64, 1999

Park, Shigenaga, Degan, Korn, Kitzler, Wehr, Kolachana, Ames: "Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 8, pp. 3375-9, 1992