CRH antibody

Catalog No. ABIN111808

Product Details anti-CRH Antibody

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRH antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific for corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland. The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.Aff. Const.:10(e)-9 M with native and modified CRF

Purification: Affinity chromatography on Protein G

Immunogen: Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.

Clone: 4H9

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen sections. Immunohistochemistry on Paraffin sections: 1/100 - 1/7000, Microwave pretreatment isrequired. Enzyme digestion with 0.1 % trypsin may also be necessary. ELISA: This antibody is suitable for use in a sandwich ELISA with clone 5D5 (BM356).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Alternative Name: Corticoliberin (CRH Products)

Synonyms: CRF antibody, Gm1347 antibody, crf antibody, crh antibody, zgc:101864 antibody, CRH antibody, corticoliberin antibody, crhb antibody, crh.1-a antibody, crha antibody, corticotropin releasing hormone antibody, corticotropin releasing hormone b antibody, corticotropin releasing hormone S homeolog antibody, corticotropin releasing hormone L homeolog antibody, Crh antibody, CRH antibody, crhb antibody, crh antibody, crh.S antibody, crh.L antibody

Background: CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).Synonyms: CRF, CRH, Corticotropin-Releasing Factor, Corticotropin-Releasing Hormone

Gene ID: 1392

NCBI Accession: NP_000747

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour