Intercellular Adhesion Molecule 1 (ICAM1) antibody (Biotin)

Details for Product No. ABIN111935
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Antigen
Synonyms BB2, CD54, P3.58, Icam-1, Ly-47, MALA-2, ICAM1, ICAM-1, ICAM
Reactivity
Human
(488), (131), (93), (44), (35), (20), (17), (3), (2), (2)
Host
Mouse
(301), (236), (53), (5), (5), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(56), (49), (36), (10), (10), (9), (9), (8), (8), (8), (8), (8), (8), (7), (4), (4), (4), (4), (2), (2), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(331), (236), (149), (117), (103), (91), (79), (73), (48), (31), (29), (11), (10), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN111935
Quantity 0.1 mg
Price
544.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Cultured Human endothelial cells
Clone CA3
Isotype IgG2a
Specificity Clone 1304 detects CD54 (ICAM-1) and is useful for the detection of activated endothelial cells and other activated hemopoietic cells. The antibody inhibits the binding of NK-cells to target cells (K562). Intercellular adhesion molecule (ICAM) 1 or CD54 was initially identified with monoclonal antibody RR1/1 as a
Purification Protein A Chromatography.
Alternative Name CD54 / ICAM1
Background ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
Alternate names: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor
NCBI Accession 9606
UniProt P05362
Application Notes Immunohistochemistry on Frozen Sections: 0.25 µg/ml (1/800). Immunohistochemistry on Paraffin Sections: 2 µg/ml (1/100). Proteinase K pretreatmentfor antigen retrieval is recommended. Suggested Positive Control: Human tonsil. Has been described to work in FACS and Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 0.5 mL distilled water.
Concentration 0.2 mg/mL
Buffer Stock Solution contains: PBS, pH 7.2 with 0.01% Thimerosal as preservative and 1% BSA as stabilizer.
Preservative Thimerosal (Merthiolate)
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted Prior to and following reconstitution at 2-8°C. DO NOT FREEZE.
Expiry Date 12 months
Background publications Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).

Freudenthal, Steinman: "The distinct surface of human blood dendritic cells, as observed after an improved isolation method." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 19, pp. 7698-702, 1990 (PubMed).

Albelda, Buck: "Integrins and other cell adhesion molecules." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 4, Issue 11, pp. 2868-80, 1990 (PubMed).

Rothlein, Dustin, Marlin et al.: "A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 4, pp. 1270-4, 1986 (PubMed).

Hosts (301), (236), (53), (5), (5), (3), (1)
Reactivities (488), (131), (93), (44), (35), (20), (17), (3), (2), (2)
Applications (331), (236), (149), (117), (103), (91), (79), (73), (48), (31), (29), (11), (10), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Conjugates (56), (49), (36), (10), (10), (9), (9), (8), (8), (8), (8), (8), (8), (7), (4), (4), (4), (4), (2), (2), (1), (1), (1)
Epitopes (34), (12), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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