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hCG antibody

hCG Reactivity: Human EIA, IHC (p) Host: Mouse Monoclonal h6 unconjugated
Catalog No. ABIN111952
  • Target See all hCG Antibodies
    hCG (Chorionic Gonadotropin (hCG))
    Reactivity
    • 32
    • 1
    • 1
    Human
    Host
    • 19
    • 5
    • 5
    • 3
    • 1
    Mouse
    Clonality
    • 19
    • 12
    Monoclonal
    Conjugate
    • 30
    • 3
    • 1
    This hCG antibody is un-conjugated
    Application
    • 20
    • 10
    • 8
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This Monoclonal antibody Clone h6 recognizes β-HCG, a well defined oncofetal protein of 36.7 kDa m.w. associated with pregnancy and some cancers. Raised serum levels for HCG are found in teratogenic carcinomas and trophoblastic tumors and occasionally in gastrointestinal, urogenital, bronchial or mammary carcinomas, and in lymphomas.
    Purification
    Affinity Chromatography
    Clone
    H6
    Isotype
    IgG1
    Top Product
    Discover our top product hCG Primary Antibody
  • Application Notes
    Suitable for Immunohistochemistry (IHC) on: Frozen sections: 0.1 μg/mL (1/4000)Paraffin sections: 0.1 μg/mL (1/4000), microwave pretreatment for antigen retrieval isrecommended. It works also in ELISA. Suggested positive control: Human placenta.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.5 mL distilled water.
    Concentration
    0.4 mg/mL
    Buffer
    PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Target
    hCG (Chorionic Gonadotropin (hCG))
    Abstract
    hCG Products
    Synonyms
    CGB antibody, AW108023 antibody, HCG antibody, chorionic gonadotropin, beta polypeptide antibody, ubiquitin-like 3 antibody, chorionic gonadotropin beta subunit 5 antibody, CGB antibody, Ubl3 antibody, CGB5 antibody
    Background
    Human gonadothropin is a glycoprotein of 36.7 kDa consisting of two subunits. The α-chain (MW 14.5 kD) is common to three other glycoprotein hormones (TSH, FSH, LH) whereas the β-chain (22.2kD) is more different. The β-chain of HCG and LH (lutrophin) have sequence homology in the first 112 amino acids and differ at the C-terminal end consisting of 24 amino acids.Synonyms: CGB, CGB3, Choriogonadotropin subunit beta, beta hCG, hCG-beta
    Gene ID
    1082
    NCBI Accession
    NP_000728
    UniProt
    P01233
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