IL1RN antibody

Catalog No. ABIN112012

This Mouse Monoclonal antibody specifically detects IL1RN in EIA. It exhibits reactivity toward Human.

Quick Overview for IL1RN antibody (ABIN112012)

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL1RN antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Clone: 1384-92

Product Details anti-IL1RN Antibody

Purification: Affinity Chromatography.

Immunogen: Recombinant human IL-1Ra.

Isotype: IgG1

Application Details

Application Notes: Suitable for ELISA. Distribution: The antigen is produced and secreted by monocytes and macrophages afterstimulation with pro-inflammatory cytokines, LPS, and in vitro by immunoglobulins coated

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water (stock solution).

Concentration: 0.4 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2 containing 0.01 % Thimerosal as a preservative and no stabilizer.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: -20 °C

Target Details for IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL1RN / IL1RA

Background: The interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring molecule that shares homology with IL-1α and IL-1β. It binds competitively to IL-1 receptors Type I and Type II with the same affinity as does IL-1, but does not induce any intracellular response. Two structural variants of IL-1Ra have been described: a 17 kDa form that is secreted from monocytes, macrophages, neutrophils, and other cells (sIL-1Ra) and an 18- kDa form that remains in the cytoplasm of keratinocytes and other epithelial cells, monocytes, and fibroblasts (icIL-1Ra). An additional 16- kDa intracellular isoform of IL-1Ra has recently been described in neutrophils, monocytes, and hepatic cells. Both of the major isoforms of IL-1Ra are transcribed from the same gene through the use of alternative first exons. The production of IL-1Ra is stimulated by many substances including adherent IgG, other cytokines, and bacterial or viral components. The tissue distribution of IL-1Ra in mice indicates that sIL-1Ra is found predominantely in peripheral blood cells, lungs, spleen, and liver, while icIL-1Ra is found in large amounts in skin. Studies in transgenic and knockout mice indicate that IL-1Ra is important in host defense against endotoxin-induced injury. IL-1Ra is produced by hepatic cells with the characteristics of an acute phase protein. Endogenous IL-1Ra is produced in numerous experimental animal models of diesease as well as in human autoimmune and chronic inflammatory diseases. The use of neutralizing anti-IL-1Ra antibodies has demonstrated that endogenous IL-1Ra is an important natural antiinflammatory protein in arthritis, colitis, and granulomatous pulmonary disease.Synonyms: ICIL-1RA, IL-1RN, IL-1ra, IL1 inhibitor, IL1F3, IRAP, Interleukin-1 receptor antagonist protein

Gene ID: 9606

UniProt: P18510

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints