Keratin 5 (KRT5) antibody

Details for Product No. ABIN112167
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Antigen
Synonyms ckii, wu:fb02h09, wu:fj02d02, wu:fk78b12, zfCKII, KRT75, CK5, DDD, DDD1, EBS2, K5, KRT5A, 3300001P10Rik, AW146334, Krt2-5, Tfip8, Sak57, CK-5, Keratin-5
Reactivity
Amphibian, Cow (Bovine), Human, Hydra, Mouse (Murine), Pig (Porcine)
(158), (50), (36), (17), (16), (15), (4), (2), (2), (2), (2), (1), (1)
Host
Mouse
(98), (68), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(125), (82), (71), (53), (25), (24), (24), (21), (20), (10), (6), (6), (3), (1)
Pubmed 3 references available
Quantity 50 μg
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Catalog No. ABIN112167
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Immunogen Human keratin K8, purified from SDS PAGE gel
Clone C22
Isotype IgG1
Specificity Antibody clone C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia. Polypeptide reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8, formerly also designated cytokeratins 5 and 8) of human epithelial cells. Epitope has been mapped to aa 353-367 on alpha helical rod domain (see Waseem et al., 2004). Reactivities on cultured cell lines (tested so far): MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK.
Cross-Reactivity (Details) Also reacts to: KRT8
Purification Affinity Chromatography on Protein A.
Alternative Name Cytokeratin 5
Background Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5
Gene ID 3852
NCBI Accession NP_000415
UniProt P13647
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Western Blot. Immunocytology. Immunohistochemistry on Frozen Tissues (1/10), Incubation time: 1 h at RT. Immunohistochemistry on Paraffin-Embedded Tissues (1/10), Incubation time: overnight at2-8 °C. With paraffin-embedded sections, protease pretreatment is required prior toantibody application.
Restrictions For Research Use only
Reconstitution Restore in 1 mL distilled water.
Buffer Final solution contains 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer in PBS buffer, pH 7.4.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light. DO NOT FREEZE!
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C.
Background publications Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Waseem, Karsten, Leigh et al.: "Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability." in: Biochemistry, Vol. 43, Issue 5, pp. 1283-95, 2004 (PubMed).

General Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

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