Cytokeratin 5 antibody (Middle Region)

Catalog No. ABIN7602992

The Mouse Monoclonal anti-Cytokeratin 5 antibody (Clone 5D3F7) (ABIN7602992) specifically detects Cytokeratin 5 in WB, IHC, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for Cytokeratin 5 antibody (Middle Region) (ABIN7602992)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 5D3F7

Product Details anti-Cytokeratin 5 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Cytokeratin 5 Antibody (monoclonal, 5D3F7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by one amino acid,and identical to the related rat sequence.

Characteristics: Anti-Cytokeratin 5 Antibody.(monoclonal, 5D3F7)-6. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: KRT5

Background:

Background: Cytokeratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Gene Full Name: keratin 5

Molecular Weight: 62 kDa

Gene ID: 3852

UniProt: P13647