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alpha 1 Cardiac Muscle Actin / ACTC1 antibody

Details for Product No. ABIN112213, Supplier: Log in to see
Antigen
Reactivity
Chicken, Cow (Bovine), Human, Rabbit
3
2
1
Host
Mouse
3
Clonality (Clone)
Monoclonal ()
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
3
3
2
1
Supplier
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Immunogen Synthetic NH2 terminus decapeptide of cardiac isoform of Actin.
Clone AC1-20-4-2
Isotype IgG1
Specificity This antibody is specific for Fetal (Cardiac) isoform of Actin. Clone Ac1 represents an excellent marker for cardiac tissue. It discriminates fetal (cardiac) alpha-Actin from all other Actin isoforms. Mab Ac1-20.4.2 shows no cross reaction with other Actin isoforms present in skeletal and smooth muscle, provided that stringent conditions have been applied (see Special Treatment).
Cross-Reactivity (Details) Species reactivity (tested):Human, Bovine, Rabbit and Chicken.
Purification Affinity Chromatographyon Protein G.
Background Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). Fetal Actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord.
Gene ID 70
NCBI Accession NP_005150
UniProt P68032
Application Notes ELISA: 1/500. Western Blot: 1/1000 (using the ECL-enhanced procedure). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 (After microwave treatment). Incubation time: 1 h at RT. Special Treatment: It is necessary to include 0.5 M NaCl in all washing buffers forapplication on Frozen tissue and 1 M NaCl for Western blotting and ELISA to enhancespecifity. This is not necessary for Paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 1.0 mL distilled water.
Buffer Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Product cited in: Skwarek-Maruszewska, Hotulainen, Mattila, Lappalainen: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." in: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, 2009

Itzhaki-Alfia, Leor, Raanani, Sternik, Spiegelstein, Netser, Holbova, Pevsner-Fischer, Lavee, Barbash: "Patient characteristics and cell source determine the number of isolated human cardiac progenitor cells." in: Circulation, Vol. 120, Issue 25, pp. 2559-66, 2009

Background publications Moll, Holzhausen, Mennel, Kuhn, Baumann, Taege, Franke: "The cardiac isoform of alpha-actin in regenerating and atrophic skeletal muscle, myopathies and rhabdomyomatous tumors: an immunohistochemical study using monoclonal antibodies." in: Virchows Archiv : an international journal of pathology, Vol. 449, Issue 2, pp. 175-91, 2006

Ehler, Fowler, Perriard: "Myofibrillogenesis in the developing chicken heart: role of actin isoforms and of the pointed end actin capping protein tropomodulin during thin filament assembly." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 229, Issue 4, pp. 745-55, 2004

Rudy, Yatskievych, Antin, Gregorio: "Assembly of thick, thin, and titin filaments in chick precardiac explants." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 221, Issue 1, pp. 61-71, 2001

Franke, Stehr, Stumpp, Kuhn, Heid, Rackwitz, Schnoelzer, Baumann, Holzhausen, Moll: "Specific immunohistochemical detection of cardiac/fetal alpha-actin in human cardiomyocytes and regenerating skeletal muscle cells." in: Differentiation, research in biological diversity, Vol. 60, Issue 4, pp. 245-50, 1996