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Antibody
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anti-Cytokeratin 3 - Cytokeratin K2p
Cytokeratin 3 / Cytokeratin K2p antibody
| Antigen |
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| Clonality |
Monoclonal (AE5) |
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Host
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Mouse
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Reactivity
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Cow (Bovine), Human, Rabbit
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Application
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Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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| Catalog no. |
ABIN112318 |
| Quantity |
0.2 mg (1.0 mg/ml) |
| Price |
430.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: keratin-3, keratin-76, KRT3, Type-II keratin Kb3, Keratin, type II cytoskeletal 3, 65 kDacytokeratin
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Gene ID
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3850
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Swiss-Prot
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P12035
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Immunogen
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Human epithelial keratin
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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AE5
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Description
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Cytokeratin 3 belongs to the intermediate filament family. It is a type II cytokeratin and isspecifically expressed in the corneal epithelium. Cytokeratin 3 associates with Cytokeratin12. Defects in Cytokeratin 3 are a cause of Meesmann corneal dystrophy.
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Specificity
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Cytokeratin 3 antibody, clone AE5 represents an excellent marker for corneal typedifferentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has beenused for studying corneal epithelial stem cells. Polypeptide Reacting: Mr 64 000 polypeptide (Keratin K3, formerly also designatedCytokeratin 3) of human corneal epithelium and Keratin K76 (formerly also designatedCytokeratin K2p) of palate epithelium. Reactivities on Cultured Cell Lines: Rabbit corneal epithelial cells. In Cow and Rabbit it reacts with lip and snout epithelia. Species Reactivity: Tested: Human, Rabbit and Bovine.
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Application Notes
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Immunoblotting: 1/500. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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PBS, pH 7,2 containing 0.09% Sodium Azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Publications
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Cooper, Schermer, Sun: "Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 52, Issue 3, pp. 243-56, 1985 (PubMed).
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