Keratin 7 (KRT7) antibody

Antigen: Keratin 7 (KRT7)

Synonyms: K7, CK7, SCL, K2C7, MGC3625, MGC129731, Krt2-7, MGC11625, D15Wsu77e, KRT7, MGC137771

Clonality: Monoclonal (RCK105)

Host: Mouse

Reactivity: Dog (Canine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN112349

Additional Information

Alternative name: Cytokeratin 7

UniProt: P08729

Immunogen: Cytokeratins from the human bladder carcinoma cell line T24.

Cross-Reactivity: Dog (Canine), Cat (Feline), Hamster, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Isotype: IgG1

Clone: RCK105

Description: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by aremarkable biochemical diversity, represented in human epithelial tissues by at least 20different polypeptides. They range in molecular weight between 40 kDa and 68 kDa andisoelectric pH between 4. 9-7. 8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not onlycharacteristic of the type of epithelium, but also related to the degree of maturation ordifferentiation within an epithelium. Cytokeratin subtype expression patterns are used toan increasing extent in the distinction of different types of epithelial malignancies. Thecytokeratin antibodies are not only of assistance in the differential diagnosis of tumorsusing immunohistochemistry on tissue sections, but are also a useful tool in cytopathologyand flow cytometric assays.

Characteristics: Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin, Keratin type II cytoskeletal 7

Specificity: RCK105 reacts exclusively with Cytokeratin 7 which is present in a subgroup of glandularepithelia and their tumors, as well as transitional epithelium and transitional carcinoma. Species: Human, Mouse, Rat, Hamster, Canine, Swine and Feline. Others not tested.

Application Details

Application Notes: Suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry on FrozenSections and Flow Cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100- 1/1000 for Immunoblotting applications. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS with 0.09% Sodium Azide as preservative.

Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Research Area: Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix

Restrictions: For Research Use only

Publications

Ivanyi, Minke, Hageman et al.: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, 1992 (PubMed).

Bauwens, De Groot, Ramaekers et al.: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky et al.: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Schaafsma, Ramaekers, van Muijen et al.: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).