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CD4 antibody (Biotin)

CD4 Reactivity: Mouse FACS Host: Rat Monoclonal CT-CD4 Biotin
Catalog No. ABIN114145
  • Target See all CD4 Antibodies
    CD4
    Reactivity
    • 355
    • 199
    • 113
    • 38
    • 24
    • 20
    • 19
    • 18
    • 15
    • 11
    • 10
    • 10
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 328
    • 146
    • 108
    • 4
    • 2
    • 1
    • 1
    Rat
    Clonality
    • 445
    • 138
    • 3
    Monoclonal
    Conjugate
    • 195
    • 65
    • 54
    • 46
    • 30
    • 12
    • 11
    • 10
    • 9
    • 9
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This CD4 antibody is conjugated to Biotin
    Application
    • 470
    • 193
    • 99
    • 93
    • 81
    • 76
    • 71
    • 42
    • 39
    • 37
    • 33
    • 24
    • 10
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    • 6
    • 6
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    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    This CT-CD4 monolconal antibody (mAb) recognizes mouse CD4 (L3T4).
    Cross-Reactivity (Details)
    Species reactivity (tested):Mouse.
    Clone
    CT-CD4
    Isotype
    IgG2a
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    Discover our top product CD4 Primary Antibody
  • Application Notes
    Flow Cytometry (see Protocols). (Reported to be useful in immunohistochemistry on acetone fixed frozen sections)
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0-0. 5 µg of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of (Streptavidin-PE) at a 1: 20 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. MEDIA: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS containing 0.09 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CD4
    Alternative Name
    CD4 (CD4 Products)
    Synonyms
    cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
    Background
    CD4 (L3T4) is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells including Th cells1,2. Mouse CD4 has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors2,3. As a coreceptor in the TCR complex, CD4 is involved in T cell activation through interaction with MHC class II on APC's and in signal transduction via protein tyrosine kinase lck1.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
    Gene ID
    12504
    NCBI Accession
    NP_038516
    UniProt
    P06332
    Pathways
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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