IL8
Reactivity: Human
WB, ELISA, IHC (p), IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Reacts with human IL-8 (matched pair with clone I8-60, DM1023). Neutralizing: In chemotaxis assay, the antibody inhibited 97.8 % % of the chemotacticactivity of IL-8 on RB/293 cell using 5 μg/mL of MAb and 10 ng/mL of human IL-8 in assay. This MAb inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP1-beta and RANTES in neutrophil chemotaxis assay. It indicates the MAb is specialneutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calcium ion changes in humangranulocytes using FACS. Western Blot: Concentration of 0.02-0.1 μg/mL MAb will allow visualization of 100 ng/laneof human IL-8. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Double distillated water is recommended to adjust the final concentration to 1.0 mg/mL.
Buffer
0.01 M PBS, pH 7.0
Preservative
Azide free
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2-8 °C for one month or at -20 °C for longer.
Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor