Clusterin antibody

Catalog No. ABIN114700

Product Details anti-Clusterin Antibody

Target: Clusterin (CLU)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The anti-Clusterin Antibody, Clone Hs-3 is a Mouse monoclonal antibody against Human Clusterin. Does not react with Cat, Dog, Bovine.

Purification: Affinity chromatography on protein A followed by DEAE-chromatography

Immunogen: The antibody was prepared against Triton X-100 human sperm extract.

Clone: Hs-3

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Concentration: 1.0 mg/mL

Buffer: 0.05 M Phosphate buffer, 0.5 M NaCl, pH 7.2, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Expiry Date: 6 months

References for anti-Clusterin antibody (ABIN114700)

Steinke, Dally, Friedel, Walles, Walles: "Host-integration of a tissue-engineered airway patch: two-year follow-up in a single patient." in: Tissue engineering. Part A, Vol. 21, Issue 3-4, pp. 573-9, (2015) (PubMed).

Schlötzer-Schrehardt, Pasutto, Sommer, Hornstra, Kruse, Naumann, Reis, Zenkel: "Genotype-correlated expression of lysyl oxidase-like 1 in ocular tissues of patients with pseudoexfoliation syndrome/glaucoma and normal patients." in: The American journal of pathology, Vol. 173, Issue 6, pp. 1724-35, (2008) (PubMed).

Zhang, Sha, Wood, Galindo, Garner, Burkart, Suarez, Sierra, Agar, Peterson, Chopra: "Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA)." in: Cellular signalling, Vol. 20, Issue 5, pp. 844-61, (2008) (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).

Schenke-Layland, Madershahian, Riemann, Starcher, Halbhuber, König, Stock: "Impact of cryopreservation on extracellular matrix structures of heart valve leaflets." in: The Annals of thoracic surgery, Vol. 81, Issue 3, pp. 918-26, (2006) (PubMed).

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Apolipoprotein J (Apo J) (CLU Products)

Synonyms: LOC100136120 antibody, clu antibody, MGC53055 antibody, zgc:73352 antibody, xclu antibody, MGC89163 antibody, CLU antibody, APO-J antibody, APOJ antibody, CLI antibody, CLU1 antibody, CLU2 antibody, KUB1 antibody, NA1/NA2 antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, TRPM-2 antibody, TRPM2 antibody, DAG antibody, RATTRPM2B antibody, SP40 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, AI893575 antibody, ApoJ antibody, Cli antibody, D14Ucla3 antibody, Sgp-2 antibody, Sgp2 antibody, Sugp-2 antibody, GP80 antibody, gpIII antibody, 40 antibody, clusterin-1 antibody, clusterin L homeolog antibody, clusterin antibody, Clusterin antibody, LOC100136120 antibody, clu.L antibody, clu antibody, CLU antibody, clus antibody, Clu antibody, LOC101113728 antibody

Background: Clusterin is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid, but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled antiparallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha-helices and three predicted amphipathic alpha-helices. The six sites of N-linked glycosylation are indicated as yellow spots. Across a broad range of species clusterin shows 70 % to 80 % of sequence homology. It is ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A detailed mechanism of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clusterin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

Gene ID: 1191

NCBI Accession: NP_976084

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling