CCL3 antibody

Catalog No. ABIN114723

The Mouse Monoclonal anti-CCL3 antibody (Clone 125) (ABIN114723) specifically detects CCL3 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for CCL3 antibody (ABIN114723)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 125

Product Details anti-CCL3 Antibody

Specificity: Reactive with recombinant MIP-1α. Does not show any cross reaction with other human cytokines or growth factors tested such as M-CSF, GM-CSF, IL-7, IL-8, IL-16, IL-1β, bFGF, TGF-β, EGF, TNF-α and EPO.

Purification: Protein G affinity chromatography

Immunogen: Purified recombinant human MIP-1alpha

Isotype: IgG1

Application Details

Application Notes: ELISA, Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Double distillated water is recommended to adjust the final concentration to 1.00 mg/mL

Buffer: 0.01 M PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: MIP1 alpha / CCL3

Background: Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.Synonyms: C-C motif chemokine 3, CCL-3, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, LD78 alpha, MIP-1-alpha, MIP1A, Macrophage Inflammatory Protein 1 alpha, SCYA3, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Gene ID: 6348

NCBI Accession: NP_002974

UniProt: P10147

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy