His Tag antibody

Catalog No. ABIN115691

The Rabbit Polyclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB.

Quick Overview for His Tag antibody (ABIN115691)

Target: His Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Purification: Antigen Affinity Chromatography using a His-peptide as matrix.

Immunogen: Different highly purified 6x His-tagged proteins (C-terminal) produced in insect cells.

Application Details

Application Notes: Western blot (0.5-1 μg/mL): The antibody was tested using several His-tagged proteinsexpressed in insect cells and E. coli.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of > 0.5 mg/mL.

Buffer: PBS, pH 7.4, without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized IgG is stable at 2-8 °C for one month from despatch and for one year when kept at -20 °C. The reconstituted antibody can be stored at 2-8 °C for one month or at -20 °C for one year.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Alternative Name: 6xHistidine Epitope Tag

Target Type: Tag

Background: In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called