CCL7 antibody

Catalog No. ABIN115768

This Mouse Monoclonal antibody specifically detects CCL7 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CCL7 antibody (ABIN115768)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL7 Antibody

Specificity: Recognizes Human Macrophage Chemotactic Protein-3 (MCP-3)

Purification: Affinity Chromatography on Protein A

Immunogen: Recombinant Hunan MCP-3.

Isotype: IgG1

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman MCP-3 (100 ng/mL), a concentration of 1-3 μg/mL of this antibody is required. ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL ofthis antibody will detect recombinant Human MCP-3 when used with Biotin conjugatedanti-Human MCP-3 antibody (cat. PP1046B) as the detection antibody at a concentration ofapproximately 0.5-1.0 μg/mL. Western Blot: To detect Human MCP-3 by Western Blot analysis this antibody can be usedat a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human MCP-3 is 2.0-4.0 ng/lane, underreducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: MCP3 / CCL7

Background: This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7

Gene ID: 6354

NCBI Accession: NP_006264

UniProt: P80098