CCL8 antibody

Catalog No. ABIN115779

The Mouse Monoclonal anti-CCL8 antibody (ABIN115779) specifically detects CCL8 in WB, EIA and Func. The antibody is reactive with Human samples.

Quick Overview for CCL8 antibody (ABIN115779)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL8 Antibody

Specificity: Reacts with Human Macrophage Chemotactic Protein-2 (MCP-2)

Purification: Affinity Chromatography on Protein A

Immunogen: Highly pure (>98%) recombinant Human MCP-2

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant human MCP-2 when used withBiotin anti-Human MCP-2 antibody (cat. PP1047B) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human MCP-2 by Western Blot analysis this antibody can be usedat a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, undernon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman MCP-2 (100 ng/mL), a concentration of 0.8-1.0 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: MCP2 / CCL8

Background: MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.Synonyms: C-C motif chemokine 8, CCL-8, HC14, MCP-2, Monocyte Chemotactic Protein-2, Monocyte chemoattractant protein 2, SCYA10, SCYA8, Small-inducible cytokine A8

Gene ID: 6355

NCBI Accession: NP_005614

UniProt: P80075