CCL7 antibody

Catalog No. ABIN115897

This Goat Polyclonal antibody specifically detects CCL7 in WB, EIA and Func. It exhibits reactivity toward Mouse.

Quick Overview for CCL7 antibody (ABIN115897)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL7 Antibody

Specificity: Reacts with Macrophage Chemotactic Protein-3 (MCP-3)

Purification: Affinity Chromatography employing an immobilized Murine MCP-3 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Murine MCP-3.

Application Details

Application Notes: Neutralization. ELISA: To detect mMCP-3 by direct ELISA (using 100 l/well antibody solution) a concentration ofat least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mMCP-3. Western Blot: To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: MCP3 / CCL7

Background: This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7

Gene ID: 20306

NCBI Accession: NP_038682

UniProt: Q03366