CXCL1 antibody

Catalog No. ABIN115941

The Rabbit Polyclonal anti-CXCL1 antibody (ABIN115941) specifically detects CXCL1 in WB, EIA and Func. The antibody is reactive with Rat samples.

Quick Overview for CXCL1 antibody (ABIN115941)

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CXCL1 Antibody

Specificity: Recognises Rat GRO/KC.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant rGRO/KC.

Application Details

Application Notes: ELISA: To detect rGRO/KC by direct ELISA (using 100 l/well antibody solution) a concentration ofat least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody allowsthe detection of 0.2-0.4 ng/well of recombinant rGRO/KC. Western Blot: To detect rGRO/KC this antibody can be used at a concentration of 0.1-0.2 μg/mL. Thedetection limit for recombinant rGRO/KC is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Neutralisation: To yield one-half maximal inhibition of the biological activity of rat GRO/KC (100 ng/mL) aconcentration of 5-10 μg/mL of this antibody is requirted.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL1

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: CXCL1 / GRO-alpha

Background: The GRO gene was originally identified by subtractive hybridization studies between normal and tumorigenic Chinese hamster embryo fibroblasts. The hamster cDNA was cloned and used as a probe for cloning of the human GRO cDNA. The GROalpha gene initially cloned from T24 cells and the gene in melanoma cells encoding melanoma growth stimulating protein (MGSA) are identical. Human cells contain three closely related, but distinct GRO genes: GRO alpha, GRO beta, and GRO gamma. GRO beta and GRO gamma share 93 % and 82 % identity, respectively, with GRO alpha at the nucleotide level. GROs are members of the chemokine alpha family that is characterized by the separation with one amino acid of the first two cysteine residues, C-X-C, in the amino acid sequence. The GRO gene has been mapped to chromosome 4q21. In normal cells, human mRNA GRO expression is found in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, GRO mRNA has been detected in mammary fibroblasts, mammary epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. Characterization of the GROalpha receptor indicates the presence of low and high affinity receptors on human neutrophils.Synonyms: CXCL1, GRO, GRO-alpha(1-73), GRO1, GROA, Growth-regulated alpha protein, MGSA, Melanoma growth stimulatory activity, NAP-3, Neutrophil-activating protein 3, SCYB1

Gene ID: 81503

NCBI Accession: NP_110472

UniProt: P14095

Pathways: Autophagy