ELISA: Direct: To detect hGRO-gamma by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 1.0 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinanthGRO-gamma. Sandwich: To detect hGRO-gamma by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human GRO-gamma (PP1014P1 orPP1014P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hGRO-gamma. Western blotTo detect hGRO-gamma by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGRO-gamma is 1.5-3.0 ng/lane, under eitherrecombinant hGRO-gamma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
This product is photosensitive and should be protected from light
Growth Regulated Protein gamma (GRO gamma) is a cytokine that may play a role in inflammation and affects endothelial cells in an autocrine fashion. It belongs to the intercrine alpha family (small cytokine c-x-c) (chemokine cxc).Synonyms: C-X-C motif chemokine 3, GRO-gamma, GRO3, GROG, Growth-regulated protein gamma, MIP2-beta, Macrophage inflammatory protein 2-beta, SCYB3