IL-11 antibody (Biotin)

Catalog No. ABIN116101

This Rabbit Polyclonal antibody specifically detects IL-11 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for IL-11 antibody (Biotin) (ABIN116101)

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-11 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-11 Antibody (Biotin)

Specificity: Human Interleukin-11.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hIL-11 (human IL-11).

Application Details

Application Notes: ELISA: To detect hIL-11 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-11. Western Blot: To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIL-11 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4-8 °C for one month or at -20 °C for longer.

Target Details for IL-11

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Alternative Name: Interleukin-11 / IL11

Background: Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.Synonyms: AGIF, Adipogenesis inhibitory factor, IL-11

Gene ID: 3589

NCBI Accession: NP_000632

UniProt: P20809

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion