VEGFA antibody

Catalog No. ABIN116334

The Rabbit Polyclonal anti-VEGFA antibody (ABIN116334) specifically detects VEGFA in WB, IHC (p), EIA and Func. The antibody is reactive with Mouse samples.

Quick Overview for VEGFA antibody (ABIN116334)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-VEGFA Antibody

Specificity: Reacts with Vascular Endothelial Growth Factor (VEGF).

Purification: Affinity Chromatography.

Immunogen: E.coli derived highly pure (> 98%) recombinant Murine VEGF

Application Details

Application Notes: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (50.00 ng/mL), a concentration of 1.0-2.0 μg/mL of this antibody is required. ELISAIndirect: To detect mVEGF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant mVEGF. Sandwich: To detect mVEGF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith biotinylated Anti-Murine VEGF (PP1113B) as a detection antibody, allows the detectionof at least 0.2-0.4 ng/well of recombinant mVEGF. Western BlotTo detect mVEGF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF-A

Background: VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

Gene ID: 22339

NCBI Accession: NP_001020421

UniProt: Q00731

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling