TNFRSF1B antibody

Catalog No. ABIN116477

The Rabbit Polyclonal anti-TNFRSF1B antibody is suitable to detect TNFRSF1B in samples from Human. It has been validated for WB, IF and EIA.

Quick Overview for TNFRSF1B antibody (ABIN116477)

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-TNFRSF1B Antibody

Purification: Affinity Chromatography employing immobilized hsTNF Receptor II matrix.

Immunogen: Highly purified (>98%) Ecoli derived recombinant hsTNF Receptor II (Human soluble Tumor Necrosis Factor Receptor II).

Application Details

Application Notes: ELISA: To detect hsTNF-receptor II by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II. To detect hsTNF-receptor II by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with a biotinylated Anti-Human sTNF-receptor II (PP1222B) as adetection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinanthsTNF-receptor II. Western Blot: This antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction withcompatible secondary reagents the detection limit for recombinant hsTNF-receptor II is1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for TNFRSF1B

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Alternative Name: CD120b / TNFR2

Background: Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor

Gene ID: 9606

UniProt: P20333

Pathways: NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway